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quickblocktm western blocking buffer p0252  (Beyotime)


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    Beyotime quickblocktm western blocking buffer p0252
    Quickblocktm Western Blocking Buffer P0252, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblocktm+western+blocking+buffer+p0252/quickblocktm+blocking+buffer+for+western+blot/10__1016_slash_j__reth__2024__12__001-99-4-9
    Average 90 stars, based on 1 article reviews
    quickblocktm western blocking buffer p0252 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis
    Article Snippet: Subsequently, proteins were separated on 10% SDS-PAGE gels and transferred onto PVDF membranes (R9A84148, Millipore). .. The membranes were then blocked in QuickBlockTM Western Blocking Buffer (P0252, Beyotime) for 30 minutes and incubated overnight at 4°C with primary antibodies against TRAM1 (18243-1-AP, Proteintech) and TXNIP (12705-1-AP, Proteintech). ..

    Article Title: Increased Expression of Kv10.2 in the Hippocampus Attenuates Valproic Acid-Induced Autism-Like Behaviors in Rats.
    Article Snippet: The role of potassium channels provides suggestive evidence for the etiology of autism.. The voltage-gated potassium channel Kv10.2 (KCNH5) is widely expressed in the brain.. However, the inherent relationship between Kv10.2 and autism is still unclear.

    Article Title: The role of SFRP1 in human dermal papilla cell growth and its potential molecular mechanisms as a target in regenerative therapy
    Article Snippet: .. Membranes were soaked in QuickBlockTM Western blocking buffer (P0252, Beyotime) for 50 min, then washed three times with a TBS-T buffer, and incubated overnight at 4 °C with primary antibodies against β-actin (Servicebio), SFRP1 (Abcam), β-catenin (Cell Signaling Technology) and TERT (Abcam). .. The next day, membranes were washed with TBST again 3 times and treated with goat anti-rabbit IgG (Proteintech) for at least 1 h. Reactive proteins were visualized by Ultrasensitive ECL Chemiluminescent Substrate Kit (Biosharp).

    Article Title: Falcarindiol improves functional recovery and alleviates neuroinflammation after spinal cord injury by inhibiting STAT/MAPK signaling pathways.
    Article Snippet: .. After incubation with 5% skim-milk solution for 2 h or quickblockTM western blocking buffer (P0252, Beyotime, China) for 30 min at room temperature, the membrane was exposed to specific primary antibodies including anti-GAPDH (1:1000, 380626, zenbio, China), anti-Toll4 (1:1000, 505258, zenbio, China), anti-STAT1 (1:1000, 385799, zenbio, China), anti-P-STAT1 (1:1000, 340797, zenbio, China), anti-STAT3 (1:1000, 10253-2-AP, ProteinTech, USA), anti-P-STAT3 (1:1000, 381552, zenbio, China), anti-iNOS (1:1000, 340668, zenbio, China), anti-P-P38 (1:1000, 310091, zenbio, China), anti-P38 (1:1000, 200782, zenbio, China), anti-P-JNK (1:1000, AF3318, Affinity, USA), anti-JNK (1:1000, R24780, zenbio, China), anti-ERK (1:1000, R22685, zenbio, China), anti-P-ERK (1:1000, 301245, zenbio, China), anti-IL6 (1:1000, 347023, zenbio, China), anti-IL1β (1:1000, 26048-1-AP, ProteinTech, USA), anti-TNF-α (1:1000, WL01581, Wanleibio, China), anti-Bcl-2 (1:1000, 26593-1-AP, ProteinTech, USA), anti-Bax (1:1000, 50599-2- Ig, ProteinTech, USA), anti-Cleaved Caspase-3 (1:1000, 9661s, CST, USA) at 4 ◦C overnight. ..

    Article Title: Mitophagy activation by rapamycin enhances mitochondrial function and cognition in 5×FAD mice.
    Article Snippet: The proteins (20 μg) were initially segregated on 8–12% (w/v) sodium dodecyl sulfate polyacrylamide gels, followed by their transfer onto polyvinylidene difluoride membranes using a Trans-Blot Turbo (BioRad, Singapore). .. The membranes were blocked with QuickblockTM Western blocking buffer (cat. P0252, Beyotime Biotechnology) for 30 min, then subjected to an overnight incubation at 4 ◦C with various primary antibodies at a 1:1000 dilution: β-actin (cat. A2103, Abcam), anti-synapsin (cat. 2312 S, CST) and PSD95 (cat. GB11277, Servicebio), OPA1 (cat. 80741 S, CST) and MFN2 (cat. 11925 S, CST) and DRP1 (cat. 8570 S, CST), Phospho-DRP1(ser637) (cat. 4867 S, CST) and PINK1 (cat. 23274–1-AP, Proteintech) and Parkin (cat. ab77924, Abcam), LC3 (cat. SAB5701328, Sigma), Beclin-1 (cat. 207612, Abcam) and mTOR (cat. 2972 S, CST), and SQSTM1/p62 (cat. ab109012, Abcam). .. The membranes were then washed 3 times in 0.1% TBS/Tween-20 and incubated with an anti-rabbit secondary antibody (cat. 7074 S, CST) at room temperature for 2 h. Finally, the bands were visualized using a ChemiDoc imaging system (Bio-Rad), and quantitative analysis was conducted using ImageJ software.

    Article Title: Circulating LPS from gut microbiota leverages stenosis-induced deep vein thrombosis in mice.
    Article Snippet: Extracted proteins were quantified using a BCA Protein Assay kit (Cat# 23,227, Thermo Fisher Scientific), and the samples were loaded onto SDS-PAGE using 4–20% gradient gels (Cat# M00656, GenScript Biotech), followed by transfer to a PVDF membrane (Cat# ISEQ00010, Merck Millipore). .. After the transfer, the membrane was blocked with QuickBlockTM Western Blocking Buffer (Cat# P0252, Beyotime Biotech) for 1 h at room temperature and then used for immunoblotting. .. Antibodies used in study included an anti-occludin antibody (Cat# ab167161, Abcam), an anti-ZO-1 antibody (Cat# 21773- 1-AP, Proteintech), and an anti-β-actin antibody (Cat# 66009-1-1 g, Proteintech), an anti-TSP1 antibody (Cat# sc-59,887, Santa Cruz Biotechnology), and an anti-transferrin antibody (Cat# ab278498, Abcam).

    Article Title: The role of SFRP1 in human dermal papilla cell growth and its potential molecular mechanisms as a target in regenerative therapy
    Article Snippet: Background: Secreted frizzled-related protein 1 (SFRP1) inhibits Wnt signaling and is differentially expressed in human hair dermal papilla cells (DPCs).. However, the specific effect of SFRP1 on cell function remains unclear.. Telomerase reverse transcriptase (TERT) representing telomerase activity was found highly active around the hair dermal papilla.

    Article Title: β-arrestin2 mediates the hippocampal dopaminergic system in autistic mouse through the ERK signaling pathway.
    Article Snippet: Autism is a complex neurodevelopmental disease that may be caused by genetic and environmental factors, that are incompletely understood.. Overactivation of dopaminergic receptors can lead to autistic-like behavior. β-arrestin2 (Arrb2) is a scaffolding protein of the arrestin family, which function as cytosolic multifunctional adapter proteins that activate cell signal transduction and mediate the signal termination and endocytosis of Gprotein-coupled receptors (GPCRs) complexes.. In this study, we established an Arrb2 knockout (Arrb2 /− ) mouse to explore the biological function of Arrb2 in autistic-like behavior caused by abnormality in the dopaminergic system.

    Blocking Assay:

    Article Title: Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis
    Article Snippet: Subsequently, proteins were separated on 10% SDS-PAGE gels and transferred onto PVDF membranes (R9A84148, Millipore). .. The membranes were then blocked in QuickBlockTM Western Blocking Buffer (P0252, Beyotime) for 30 minutes and incubated overnight at 4°C with primary antibodies against TRAM1 (18243-1-AP, Proteintech) and TXNIP (12705-1-AP, Proteintech). ..

    Article Title: Increased Expression of Kv10.2 in the Hippocampus Attenuates Valproic Acid-Induced Autism-Like Behaviors in Rats.
    Article Snippet: The role of potassium channels provides suggestive evidence for the etiology of autism.. The voltage-gated potassium channel Kv10.2 (KCNH5) is widely expressed in the brain.. However, the inherent relationship between Kv10.2 and autism is still unclear.

    Article Title: The role of SFRP1 in human dermal papilla cell growth and its potential molecular mechanisms as a target in regenerative therapy
    Article Snippet: .. Membranes were soaked in QuickBlockTM Western blocking buffer (P0252, Beyotime) for 50 min, then washed three times with a TBS-T buffer, and incubated overnight at 4 °C with primary antibodies against β-actin (Servicebio), SFRP1 (Abcam), β-catenin (Cell Signaling Technology) and TERT (Abcam). .. The next day, membranes were washed with TBST again 3 times and treated with goat anti-rabbit IgG (Proteintech) for at least 1 h. Reactive proteins were visualized by Ultrasensitive ECL Chemiluminescent Substrate Kit (Biosharp).

    Article Title: Falcarindiol improves functional recovery and alleviates neuroinflammation after spinal cord injury by inhibiting STAT/MAPK signaling pathways.
    Article Snippet: .. After incubation with 5% skim-milk solution for 2 h or quickblockTM western blocking buffer (P0252, Beyotime, China) for 30 min at room temperature, the membrane was exposed to specific primary antibodies including anti-GAPDH (1:1000, 380626, zenbio, China), anti-Toll4 (1:1000, 505258, zenbio, China), anti-STAT1 (1:1000, 385799, zenbio, China), anti-P-STAT1 (1:1000, 340797, zenbio, China), anti-STAT3 (1:1000, 10253-2-AP, ProteinTech, USA), anti-P-STAT3 (1:1000, 381552, zenbio, China), anti-iNOS (1:1000, 340668, zenbio, China), anti-P-P38 (1:1000, 310091, zenbio, China), anti-P38 (1:1000, 200782, zenbio, China), anti-P-JNK (1:1000, AF3318, Affinity, USA), anti-JNK (1:1000, R24780, zenbio, China), anti-ERK (1:1000, R22685, zenbio, China), anti-P-ERK (1:1000, 301245, zenbio, China), anti-IL6 (1:1000, 347023, zenbio, China), anti-IL1β (1:1000, 26048-1-AP, ProteinTech, USA), anti-TNF-α (1:1000, WL01581, Wanleibio, China), anti-Bcl-2 (1:1000, 26593-1-AP, ProteinTech, USA), anti-Bax (1:1000, 50599-2- Ig, ProteinTech, USA), anti-Cleaved Caspase-3 (1:1000, 9661s, CST, USA) at 4 ◦C overnight. ..

    Article Title: Mitophagy activation by rapamycin enhances mitochondrial function and cognition in 5×FAD mice.
    Article Snippet: The proteins (20 μg) were initially segregated on 8–12% (w/v) sodium dodecyl sulfate polyacrylamide gels, followed by their transfer onto polyvinylidene difluoride membranes using a Trans-Blot Turbo (BioRad, Singapore). .. The membranes were blocked with QuickblockTM Western blocking buffer (cat. P0252, Beyotime Biotechnology) for 30 min, then subjected to an overnight incubation at 4 ◦C with various primary antibodies at a 1:1000 dilution: β-actin (cat. A2103, Abcam), anti-synapsin (cat. 2312 S, CST) and PSD95 (cat. GB11277, Servicebio), OPA1 (cat. 80741 S, CST) and MFN2 (cat. 11925 S, CST) and DRP1 (cat. 8570 S, CST), Phospho-DRP1(ser637) (cat. 4867 S, CST) and PINK1 (cat. 23274–1-AP, Proteintech) and Parkin (cat. ab77924, Abcam), LC3 (cat. SAB5701328, Sigma), Beclin-1 (cat. 207612, Abcam) and mTOR (cat. 2972 S, CST), and SQSTM1/p62 (cat. ab109012, Abcam). .. The membranes were then washed 3 times in 0.1% TBS/Tween-20 and incubated with an anti-rabbit secondary antibody (cat. 7074 S, CST) at room temperature for 2 h. Finally, the bands were visualized using a ChemiDoc imaging system (Bio-Rad), and quantitative analysis was conducted using ImageJ software.

    Article Title: Circulating LPS from gut microbiota leverages stenosis-induced deep vein thrombosis in mice.
    Article Snippet: Extracted proteins were quantified using a BCA Protein Assay kit (Cat# 23,227, Thermo Fisher Scientific), and the samples were loaded onto SDS-PAGE using 4–20% gradient gels (Cat# M00656, GenScript Biotech), followed by transfer to a PVDF membrane (Cat# ISEQ00010, Merck Millipore). .. After the transfer, the membrane was blocked with QuickBlockTM Western Blocking Buffer (Cat# P0252, Beyotime Biotech) for 1 h at room temperature and then used for immunoblotting. .. Antibodies used in study included an anti-occludin antibody (Cat# ab167161, Abcam), an anti-ZO-1 antibody (Cat# 21773- 1-AP, Proteintech), and an anti-β-actin antibody (Cat# 66009-1-1 g, Proteintech), an anti-TSP1 antibody (Cat# sc-59,887, Santa Cruz Biotechnology), and an anti-transferrin antibody (Cat# ab278498, Abcam).

    Article Title: The role of SFRP1 in human dermal papilla cell growth and its potential molecular mechanisms as a target in regenerative therapy
    Article Snippet: Background: Secreted frizzled-related protein 1 (SFRP1) inhibits Wnt signaling and is differentially expressed in human hair dermal papilla cells (DPCs).. However, the specific effect of SFRP1 on cell function remains unclear.. Telomerase reverse transcriptase (TERT) representing telomerase activity was found highly active around the hair dermal papilla.

    Article Title: β-arrestin2 mediates the hippocampal dopaminergic system in autistic mouse through the ERK signaling pathway.
    Article Snippet: Autism is a complex neurodevelopmental disease that may be caused by genetic and environmental factors, that are incompletely understood.. Overactivation of dopaminergic receptors can lead to autistic-like behavior. β-arrestin2 (Arrb2) is a scaffolding protein of the arrestin family, which function as cytosolic multifunctional adapter proteins that activate cell signal transduction and mediate the signal termination and endocytosis of Gprotein-coupled receptors (GPCRs) complexes.. In this study, we established an Arrb2 knockout (Arrb2 /− ) mouse to explore the biological function of Arrb2 in autistic-like behavior caused by abnormality in the dopaminergic system.

    Incubation:

    Article Title: Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis
    Article Snippet: Subsequently, proteins were separated on 10% SDS-PAGE gels and transferred onto PVDF membranes (R9A84148, Millipore). .. The membranes were then blocked in QuickBlockTM Western Blocking Buffer (P0252, Beyotime) for 30 minutes and incubated overnight at 4°C with primary antibodies against TRAM1 (18243-1-AP, Proteintech) and TXNIP (12705-1-AP, Proteintech). ..

    Article Title: Increased Expression of Kv10.2 in the Hippocampus Attenuates Valproic Acid-Induced Autism-Like Behaviors in Rats.
    Article Snippet: The role of potassium channels provides suggestive evidence for the etiology of autism.. The voltage-gated potassium channel Kv10.2 (KCNH5) is widely expressed in the brain.. However, the inherent relationship between Kv10.2 and autism is still unclear.

    Article Title: The role of SFRP1 in human dermal papilla cell growth and its potential molecular mechanisms as a target in regenerative therapy
    Article Snippet: .. Membranes were soaked in QuickBlockTM Western blocking buffer (P0252, Beyotime) for 50 min, then washed three times with a TBS-T buffer, and incubated overnight at 4 °C with primary antibodies against β-actin (Servicebio), SFRP1 (Abcam), β-catenin (Cell Signaling Technology) and TERT (Abcam). .. The next day, membranes were washed with TBST again 3 times and treated with goat anti-rabbit IgG (Proteintech) for at least 1 h. Reactive proteins were visualized by Ultrasensitive ECL Chemiluminescent Substrate Kit (Biosharp).

    Article Title: Falcarindiol improves functional recovery and alleviates neuroinflammation after spinal cord injury by inhibiting STAT/MAPK signaling pathways.
    Article Snippet: .. After incubation with 5% skim-milk solution for 2 h or quickblockTM western blocking buffer (P0252, Beyotime, China) for 30 min at room temperature, the membrane was exposed to specific primary antibodies including anti-GAPDH (1:1000, 380626, zenbio, China), anti-Toll4 (1:1000, 505258, zenbio, China), anti-STAT1 (1:1000, 385799, zenbio, China), anti-P-STAT1 (1:1000, 340797, zenbio, China), anti-STAT3 (1:1000, 10253-2-AP, ProteinTech, USA), anti-P-STAT3 (1:1000, 381552, zenbio, China), anti-iNOS (1:1000, 340668, zenbio, China), anti-P-P38 (1:1000, 310091, zenbio, China), anti-P38 (1:1000, 200782, zenbio, China), anti-P-JNK (1:1000, AF3318, Affinity, USA), anti-JNK (1:1000, R24780, zenbio, China), anti-ERK (1:1000, R22685, zenbio, China), anti-P-ERK (1:1000, 301245, zenbio, China), anti-IL6 (1:1000, 347023, zenbio, China), anti-IL1β (1:1000, 26048-1-AP, ProteinTech, USA), anti-TNF-α (1:1000, WL01581, Wanleibio, China), anti-Bcl-2 (1:1000, 26593-1-AP, ProteinTech, USA), anti-Bax (1:1000, 50599-2- Ig, ProteinTech, USA), anti-Cleaved Caspase-3 (1:1000, 9661s, CST, USA) at 4 ◦C overnight. ..

    Article Title: Mitophagy activation by rapamycin enhances mitochondrial function and cognition in 5×FAD mice.
    Article Snippet: The proteins (20 μg) were initially segregated on 8–12% (w/v) sodium dodecyl sulfate polyacrylamide gels, followed by their transfer onto polyvinylidene difluoride membranes using a Trans-Blot Turbo (BioRad, Singapore). .. The membranes were blocked with QuickblockTM Western blocking buffer (cat. P0252, Beyotime Biotechnology) for 30 min, then subjected to an overnight incubation at 4 ◦C with various primary antibodies at a 1:1000 dilution: β-actin (cat. A2103, Abcam), anti-synapsin (cat. 2312 S, CST) and PSD95 (cat. GB11277, Servicebio), OPA1 (cat. 80741 S, CST) and MFN2 (cat. 11925 S, CST) and DRP1 (cat. 8570 S, CST), Phospho-DRP1(ser637) (cat. 4867 S, CST) and PINK1 (cat. 23274–1-AP, Proteintech) and Parkin (cat. ab77924, Abcam), LC3 (cat. SAB5701328, Sigma), Beclin-1 (cat. 207612, Abcam) and mTOR (cat. 2972 S, CST), and SQSTM1/p62 (cat. ab109012, Abcam). .. The membranes were then washed 3 times in 0.1% TBS/Tween-20 and incubated with an anti-rabbit secondary antibody (cat. 7074 S, CST) at room temperature for 2 h. Finally, the bands were visualized using a ChemiDoc imaging system (Bio-Rad), and quantitative analysis was conducted using ImageJ software.

    Article Title: The role of SFRP1 in human dermal papilla cell growth and its potential molecular mechanisms as a target in regenerative therapy
    Article Snippet: Background: Secreted frizzled-related protein 1 (SFRP1) inhibits Wnt signaling and is differentially expressed in human hair dermal papilla cells (DPCs).. However, the specific effect of SFRP1 on cell function remains unclear.. Telomerase reverse transcriptase (TERT) representing telomerase activity was found highly active around the hair dermal papilla.

    Article Title: β-arrestin2 mediates the hippocampal dopaminergic system in autistic mouse through the ERK signaling pathway.
    Article Snippet: Autism is a complex neurodevelopmental disease that may be caused by genetic and environmental factors, that are incompletely understood.. Overactivation of dopaminergic receptors can lead to autistic-like behavior. β-arrestin2 (Arrb2) is a scaffolding protein of the arrestin family, which function as cytosolic multifunctional adapter proteins that activate cell signal transduction and mediate the signal termination and endocytosis of Gprotein-coupled receptors (GPCRs) complexes.. In this study, we established an Arrb2 knockout (Arrb2 /− ) mouse to explore the biological function of Arrb2 in autistic-like behavior caused by abnormality in the dopaminergic system.

    Membrane:

    Article Title: Increased Expression of Kv10.2 in the Hippocampus Attenuates Valproic Acid-Induced Autism-Like Behaviors in Rats.
    Article Snippet: The role of potassium channels provides suggestive evidence for the etiology of autism.. The voltage-gated potassium channel Kv10.2 (KCNH5) is widely expressed in the brain.. However, the inherent relationship between Kv10.2 and autism is still unclear.

    Article Title: Falcarindiol improves functional recovery and alleviates neuroinflammation after spinal cord injury by inhibiting STAT/MAPK signaling pathways.
    Article Snippet: .. After incubation with 5% skim-milk solution for 2 h or quickblockTM western blocking buffer (P0252, Beyotime, China) for 30 min at room temperature, the membrane was exposed to specific primary antibodies including anti-GAPDH (1:1000, 380626, zenbio, China), anti-Toll4 (1:1000, 505258, zenbio, China), anti-STAT1 (1:1000, 385799, zenbio, China), anti-P-STAT1 (1:1000, 340797, zenbio, China), anti-STAT3 (1:1000, 10253-2-AP, ProteinTech, USA), anti-P-STAT3 (1:1000, 381552, zenbio, China), anti-iNOS (1:1000, 340668, zenbio, China), anti-P-P38 (1:1000, 310091, zenbio, China), anti-P38 (1:1000, 200782, zenbio, China), anti-P-JNK (1:1000, AF3318, Affinity, USA), anti-JNK (1:1000, R24780, zenbio, China), anti-ERK (1:1000, R22685, zenbio, China), anti-P-ERK (1:1000, 301245, zenbio, China), anti-IL6 (1:1000, 347023, zenbio, China), anti-IL1β (1:1000, 26048-1-AP, ProteinTech, USA), anti-TNF-α (1:1000, WL01581, Wanleibio, China), anti-Bcl-2 (1:1000, 26593-1-AP, ProteinTech, USA), anti-Bax (1:1000, 50599-2- Ig, ProteinTech, USA), anti-Cleaved Caspase-3 (1:1000, 9661s, CST, USA) at 4 ◦C overnight. ..

    Article Title: Circulating LPS from gut microbiota leverages stenosis-induced deep vein thrombosis in mice.
    Article Snippet: Extracted proteins were quantified using a BCA Protein Assay kit (Cat# 23,227, Thermo Fisher Scientific), and the samples were loaded onto SDS-PAGE using 4–20% gradient gels (Cat# M00656, GenScript Biotech), followed by transfer to a PVDF membrane (Cat# ISEQ00010, Merck Millipore). .. After the transfer, the membrane was blocked with QuickBlockTM Western Blocking Buffer (Cat# P0252, Beyotime Biotech) for 1 h at room temperature and then used for immunoblotting. .. Antibodies used in study included an anti-occludin antibody (Cat# ab167161, Abcam), an anti-ZO-1 antibody (Cat# 21773- 1-AP, Proteintech), and an anti-β-actin antibody (Cat# 66009-1-1 g, Proteintech), an anti-TSP1 antibody (Cat# sc-59,887, Santa Cruz Biotechnology), and an anti-transferrin antibody (Cat# ab278498, Abcam).



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